Class documents¶

NEU466G, Dr. Harris

Overview¶

These are the PyReconstruct handouts for NEU466G, written for the current version of PyReconstruct. Lecture slides, quizzes, attendance and submissions stay on Canvas.

Module 1: Postsynaptic structure and function¶

• Lab 1.1: Dendritic Spines (Week 1)
• Lab 1.2: The Postsynaptic Density (Week 2)

Module 2: Presynaptic structure and function¶

• Lab 2.1: Presynaptic Boutons and Mitochondria (Weeks 3 and 4)

Module 3: Postsynaptic subcellular resources¶

• Lab 3.1: Smooth Endoplasmic Reticulum, Spine Apparatus, and Endosomes (Week 5)
• Lab 3.2: Polyribosomes (Week 6)

Module 4: Class research project¶

• Project overview (Weeks 7 to 14)
• Synapse categories (Weeks 7 and 8)
• Postsynaptic resources (Weeks 8 and 9)
• Data curation (Week 11)
• 3D analysis (Weeks 12 and 13)

Start here¶

• Getting started: install PyReconstruct and open the class series
• PyReconstruct Guide
• Top 10 things to learn in PyReconstruct
• Electron micrographs of the neuropil: basic literacy

Course¶

• Schedule
• Readings
• Assignments

Writing¶

• Figure legend example and tips
• Final lab report

Teaching team¶

Teaching team sign-in: opens the teaching-team pages. Teaching-team password required.